jule gradient former (Jule Inc)
90
Structured Review
Jule Inc
jule gradient former
Jule Gradient Former, supplied by Jule Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jule+gradient+former/jule+gradient+former/pm36852649-102-41-44
Average 90 stars, based on 1 article reviews
Jule Gradient Former, supplied by Jule Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jule+gradient+former/jule+gradient+former/pm36852649-102-41-44
Average 90 stars, based on 1 article reviews
jule gradient former - by Bioz Stars,
2026-09
90/100 stars
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Gradient Centrifugation:Article Title: The Upstream Conserved Regions (UCRs) Mediate Homo- and Hetero-oligomerization of Type 4 Cyclic Nucleotide Phosphodiesterases (PDE4s) Article Snippet: .. Density gradient centrifugation Density gradients from 5 to 33 % sucrose in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.2 mM EGTA, 10 mM NaF and 1.34 mM β-mercaptoethanol were prepared in 14x89-mm centrifugation tubes (Beckman Coulter Inc., Brea, CA) using the Centrifugation:Article Title: The Upstream Conserved Regions (UCRs) Mediate Homo- and Hetero-oligomerization of Type 4 Cyclic Nucleotide Phosphodiesterases (PDE4s) Article Snippet: .. Density gradient centrifugation Density gradients from 5 to 33 % sucrose in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.2 mM EGTA, 10 mM NaF and 1.34 mM β-mercaptoethanol were prepared in 14x89-mm centrifugation tubes (Beckman Coulter Inc., Brea, CA) using the Generated:Article Title: Intraflagellar Transport (IFT) Protein IFT25 Is a Phosphoprotein Component of IFT Complex B and Physically Interacts with IFT27 in Chlamydomonas Article Snippet: Flagella in the supernatant fraction were harvested by centrifugation at 14,000 rpm (Eppendorf, 5415R) for 10 minutes at 4°C. .. Linear 12 ml 10–25% sucrose density gradients in 1×HMDEK buffer plus protease inhibitors were generated by using the Article Title: Chlamydomonas RABL4/IFT27 Mediates Phototaxis via Promoting BBSome-dependent Ciliary Export of Phospholipase D Article Snippet: .. Linear 12 ml of 10-25% sucrose density gradients in 1×HMDEKN buffer plus protease inhibitors (1 mM PMSF, 50 μg/ml soybean trypsin inhibitor, 1 μg/ml pepstatin A, 2 μg/ml aprotinin, 1 μg/ml leupeptin) and 1% NP-40 were generated by using the Article Title: RABL4/IFT27 in a nucleotide-independent manner promotes phospholipase D ciliary retrieval via facilitating BBSome reassembly at the ciliary tip. Article Snippet: Funding information National Natural Science Foundation of China; China Postdoctoral Science Foundation Abstract Certain ciliary transmembrane and membrane‐associated signaling proteins export from cilia as intraflagellar transport (IFT) cargoes in a BBSome‐dependent manner.. Upon reaching the ciliary tip via anterograde IFT, the BBSome disassembles before being reassembled to form an intact entity for cargo phospholipase D (PLD) coupling.. During this BBSome remodeling process, Chlamydomonas Rab‐like 4 GTPase IFT27, by binding its partner IFT25 to form the heterodimeric IFT25/27, is indispensable for BBSome reassembly. Article Title: Bardet–Biedl syndrome 3 protein promotes ciliary exit of the signaling protein phospholipase D via the BBSome Article Snippet: .. In brief, linear 12 mL of 10–25% sucrose density gradients in 1× HMDEK buffer plus protease inhibitors and 1% NP-40 were generated using the |